nitrous oxide reductase iron

Nitrous oxide reductase

2013-1-15 · Nitrous oxide is a potent greenhouse gas, whose atmospheric concentration has been increasing since the introduction of the Haber Bosch process led to the widespread use of nitrogenous fertilizers. One of the pathways to its destruction is reduction to molecular nitrogen by the enzyme nitrous oxide reductase found in denitrifying bacteria.

Nitrous-Oxide Reductase

Nitrous oxide reductase (N 2 OR) catalyzes the last step of the dissimilatory metabolic pathway of denitrifying bacteria (e.g., Pseudomonas stutzeri ), for a recent review see Eady et al. 263 This is a two-electron reduction from N 2 O to N 2 and water according to Equation (16) (16) N 2 O + 2 e − + 2 H + → N 2 + H 2 O.

The Catalytic Center in Nitrous Oxide Reductase, CuZ, Is a ...

2000-9-30 · The crystal structure of nitrous oxide reductase, the enzyme catalyzing the final step of bacterial denitrification in which nitrous oxide is reduced to dinitrogen, exhibits a novel catalytic site, called CuZ. This comprises a cluster of four copper ions bound by seven histidines and three other ligands modeled in the X-ray structure as OH- or H2O.

Nitrite and Nitrous Oxide Reductase Regulation by …

The membrane-bound component, NosR, necessary for the expression of the nitrous oxide reductase in this strain, may be implicated in this regulation . R. sphaeroides f. sp. denitrificans IL106 is one of the few purple, nonsulfur denitrifying photosynthetic bacteria able to perform a complete denitrification process ( 16, 23, 29, 32, 35 ).

Biological sources and sinks of nitrous oxide and ...

1. Introduction. Nitrous oxide (N 2 O) is a colourless, non-toxic gas, commonly known as laughing gas. Since its discovery over 200 years ago, it has found use both as an anaesthetic and a fuel additive. However, in 1908, the invention of …

Functional assembly of nitrous oxide reductase provides ...

2019-6-25 · nitrous oxide reductase; denitrification; enzyme refactoring; cofactor biogenesis; structural biology; Nitrous oxide (N 2 O, "laughing gas") is a potent greenhouse gas whose 100-y global warming potential exceeds the one of carbon dioxide (CO 2) by a factor of 300 () s atmospheric concentration increased by ∼20% since preindustrial times and is still growing at …

nosZ

Nitrous-oxide reductase is part of a bacterial respiratory system which is activated under anaerobic conditions in the presence of nitrate or nitrous oxide. UniProtKB. x; UniProtKB. Protein knowledgebase. UniParc. Sequence archive. Help. Help pages, FAQs, UniProtKB manual, documents, news archive and Biocuration projects. ...

A functional nitric oxide reductase model | PNAS

2008-10-14 · Nitric oxide reductase (NOR) is a membrane-bound enzyme that catalyzes the 2e − reduction of nitric oxide (NO) to nitrous oxide (N 2 O), an obligatory step involved in the sequential reduction of nitrate to dinitrogen known as bacterial denitrification. The active site of NOR consists of a monohistidine ligated five-coordinate heme and a trisimidazole ligated nonheme Fe B.

Frontiers | Potential Pathway of Nitrous Oxide Formation ...

Introduction. Nitrous oxide (N 2 O) is a potent greenhouse gas, and its potential to increase global warming is approximately 300-fold higher than CO 2 (Tian et al., 2018).Globally, the primary sources that release N 2 O into the atmosphere are soil, ocean, manure application, industries, and biomass burning (Thomson et al., 2012).The nitrification and denitrification processes, …

Nitrous Oxide Reductase | SpringerLink

Nitrous oxide reductase is the last enzyme of the denitrification cycle and is the only known enzyme to catalyze in vivo the reduction of nitrous oxide to molecular nitrogen. This enzyme was first isolated in 1972, though most of its spectroscopic and kinetic properties were only determined in the late 80s and 90s, and up to now many of this ...

Unexpected nondenitrifier nitrous oxide reductase gene ...

2012-11-27 · Agricultural and industrial practices more than doubled the intrinsic rate of terrestrial N fixation over the past century with drastic consequences, including increased atmospheric nitrous oxide (N2O) concentrations. N2O is a potent greenhouse gas and contributor to ozone layer destruction, and its release from fixed N is almost entirely controlled by microbial activities.

Nitrous oxide reductase from denitrifying Pseudomonas …

2017-12-15 · Nitrous oxide (N20) respiration by the denitrifying bacterium Pseudomonas stutzeri requires the synthesis of the multi-copper enzyme N20 reductase. nosZ, the structural gene for this enzyme, is part of a DNA region of approximately 8 kbp that carries several essential genes. Insertional mutagenesis of the region downstream of

nosZ

Nitrous-oxide reductase is part of a bacterial respiratory system which is activated under anaerobic conditions in the presence of nitrate or nitrous oxide. UniRule annotation Catalytic activity i. 2 [Fe(III)cytochrome c] + H 2 O + N 2 = 2 [Fe(II)cytochrome c] + 2 H + + nitrous oxide. UniRule annotation. EC: 1.7.2.4 ...

Functional assembly of nitrous oxide reductase provides ...

Functional assembly of nitrous oxide reductase provides insights into copper site maturation Lin Zhanga, Anja Wüsta, Benedikt Prassera, Christoph Müllera, and Oliver Einslea,b,1 aInstitut für Biochemie, Albert-Ludwigs-Universität Freiburg, 79104 Freiburg, Germany; and bBIOSS Centre for Biological Signalling Studies, 79104 Freiburg, Germany Edited by Robert Huber, Max …

Purification and Some Characteristics of a Cytochrome c ...

1989-2-5 · Nitrous oxide reductase from Wolinella succinogenes was purified very nearly to homogeneity. The enzyme was found to be dimeric, with Mr = 162,000 and…

Purification, Characterization, and Preliminary ...

2019-5-26 · The aerobic purification of Pseudomonas nautica 617 nitrous oxide reductase yielded two forms of the enzyme exhibiting different chromatographic behaviors. The protein contains six copper atoms per monomer, arranged in two centers named CuA and CuZ. CuZ could be neither oxidized nor further reduced under our experimental conditions, and exhibits a 4-line EPR …

nosZ

Nitrous-oxide reductase is part of a bacterial respiratory system which is activated under anaerobic conditions in the presence of nitrate or nitrous oxide. "Nitrous oxide reductase from denitrifying Pseudomonas perfectomarina. Purification and properties of …

Nitrous oxide reductase from CuA to CuZ | Nature ...

2000-3-1 · A. to Cu. Z. The crystal structure of nitrous oxide reductase reveals a novel tetranuclear copper cluster and an unusual dimeric structure. One of the main branches of the bacterial inorganic ...

Nitrous Oxide Reductases

2007-1-1 · 5.1. Introduction. Nitrous oxide (N 2 O) is an inorganic metabolite of the prokaryotic cell during denitrification. Thus, denitrifiers comprise the principal group of N 2 O producers, with roles played also by nitrifiers, methanotrophic bacteria, and fungi. Among them, only denitrifying prokaryotes have the ability to convert N 2 O into N 2 nversion of N 2 O into N 2 is the last …

A Novel Type of Catalytic Copper Cluster in Nitrous Oxide ...

Nitrous oxide reductase (NosZ), responsible for the conversion of N2O to N2 in denitrifiers, requires copper for its activity (Brown et al., ... Iron oxide minerals are ubiquitous in soils ...

Nutrients removal and nitrous oxide emission during ...

2016-5-2 · The short- and long-term influences of ferric iron (Fe(III)) on nutrients removal and nitrous oxide (N 2 O) emission during SNDPR process were evaluated. According to the continuous cycle experiments, it was concluded that the addition of Fe(III) could lower the nitrogen removal of the following cycle during SNDPR process, which was mainly induced by the …

Unexpected nondenitrifier nitrous oxide reductase gene ...

Unexpected nondenitrifier nitrous oxide reductase gene diversity and abundance in soils Robert A. Sanford3, Darlene D. Wagnerb, Qingzhong Wuc, Joanne C. Chee-Sanfordd, Sara H. Thomas0, Claribel Cruz-Garcíac, Gina Rodríguez0,6, Arturo Massol-Deyáe, Kishore K. Krishnanif, Kirsti M. Ritalahti9''h,

Structural Basis of Biological N2O Generation by Bacterial ...

2010-12-17 · Nitric oxide reductase (NOR) is an iron-containing enzyme that catalyzes the reduction of nitric oxide (NO) to generate a major greenhouse gas, nitrous oxide (N 2 O). Here, we report the crystal structure of NOR from Pseudomonas aeruginosa at 2.7 angstrom resolution. The structure reveals details of the catalytic binuclear center.

Reduced iron induced nitric oxide and nitrous oxide ...

Here we studied the formation of nitric oxide (NO) and nitrous oxide (N (2)O) due to chemical nitrite reduction by ferrous iron (Fe (II)). Reduction of nitrite and NO coupled to Fe (II) oxidation ...

nosZ

2014-9-3 · Nitrous-oxide reductase is part of a bacterial respiratory system which is activated under anaerobic conditions in the presence of nitrate or nitrous oxide. UniRule annotation ARBA annotation. Catalytic activity i. 2 [Fe(III)cytochrome c] + H 2 O + N 2 = 2 [Fe(II)cytochrome c] + 2 H + + nitrous oxide. UniRule annotation ...

Nitrous oxide reductase from denitrifying

2017-5-4 · growth. Nitrous oxide reductase is a multicopper protein which catalyzes the transformation shown in Eqn (1). The N20 + 2e- + 2H'' +N2 + H20 (1) enzyme has a molecular mass of approximately 220 kDa and consists of two presumably identical subunits [l]. N20 is also reduced to dinitrogen by the iron-molyb-

Functional Domains of NosR, a Novel Transmembrane Iron ...

Bacterial nitrous oxide (N 2 O) respiration depends on the polytopic membrane protein NosR for the expression of N 2 O reductase from the nosZ gene. We constructed His-tagged NosR and purified it from detergent-solubilized membranes of Pseudomonas stutzeri ATCC 14405. NosR is an iron-sulfur flavoprotein with redox centers positioned at opposite sides of the cytoplasmic …

Structural basis for nitrous oxide generation by bacterial ...

to nitrous oxide as a working hypothesis. Keywords: nitric oxide reductase; denitrification; nitrous oxide generation; haem; non-haem iron; haem-copper oxidase superfamily 1. INTRODUCTION Nitric oxide reductase (NOR) is an iron-containing enzyme and catalyses the reduction of nitric oxide (NO) to nitrous oxide (N20), according to the following ...

nosZ

2003-10-3 · Nitrous-oxide reductase is part of a bacterial respiratory system which is activated under anaerobic conditions in the presence of nitrate or nitrous oxide. Catalytic activity i. 2 [Fe(III)cytochrome c] + H 2 O + N 2 = 2 [Fe(II)cytochrome c] + 2 H + + nitrous oxide. EC: 1.7.2.4. Source: Rhea. Show » « Hide] + + =] + + + ...